A method for determining alkaline phosphatase activity in marine phytoplankton using spectrofluorometry.

نویسندگان

  • Melissa Blakely Peacock
  • Raphael M Kudela
چکیده

A method for determining relative percent intensity alkaline phosphatase activity (APA) using enzyme labeled fluorescence coupled with spectrofluorometry is presented. Compared to traditional microscopy and flow cytometry, we increase statistical power and reduce sample-handling issues. Combined with a biological standard, our method can quantify APA of natural plankton assemblages.

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عنوان ژورنال:
  • Journal of microbiological methods

دوره 89 3  شماره 

صفحات  -

تاریخ انتشار 2012